The Integrative Genomics Viewer (IGV) is a widely-used, high-performance, interactive software tool designed for visualizing genomic data. Developed by the Broad Institute, IGV allows researchers to explore and interpret a wide range of genomic datasets, including DNA sequencing, RNA sequencing, and epigenomic data. It supports the analysis of alignments, variant calls, and other genomic features in a highly customizable and user-friendly interface. This lab will give you an introduction in how to use this software.

Preparations:

Before the lab you should prepare by watching some short Youtube videos and completing a quiz. You should get all questions for the quiz right (100%), the quiz can be done an unlimited amount of times so don't worry if you don't get it right on the first try :)

Videos (Appx. 25 minutes):

IGV | Data Navigation Basics (V2) (5 minutes)

IGV | Sequencing Data Basics (6 minutes)

IGV | View SNPs | Homozygous, Heterozygous, Strand Bias (3 ½ minutes)

IGV | VCF Basics | VCF File Explanation & Viewing in IGV (8 minutes)

Quiz:

The quiz can be found under "Assignments/IGV Introduction Lab".

Lab materials:

Google Drive

IGV introduction 2026.pdf

/Mikaela and Hui