ATAC-seq: data processing and peak detection

ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) is a method for determining chromatin accessibility across the genome. It utilizes a hyperactive Tn5 transposase to insert sequencing adapters into open chromatin regions. High-throughput sequencing then yields reads that indicate these regions of increased accessibility.

Figure 1. Overview of ATAC-seq (Buenrostro et al., 2015)

Learning outcomes:

After completing this tutorial you will

• understand and apply standard data processing of the ATAC-seq libraries

• be able to assess quality of the ATAC-seq libraries

• detect accessible chromatin regions in ATAC-seq data

We recommend visiting the section on ATAC-seq Links to an external site. on the course homepage for the most recent version of the course material.