Advanced ChIP techniques: overview

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a central method in epigenomic research. Since its first publication in 2007, ca 70 000 data sets obtained using ChIP-seq have been deposited in public data repositories. Recently, several new ChIP based methods have been developed, which attempt to overcome technical challenges with the original ChIP-seq protocol. We present several of these new methods in this class.

Overview of CUT&RUN technique; image source: Kaya-Okur at al, 2019

We recommend visiting the section on new ChIP methods Links to an external site. on the course homepage for the most recent version of the course material.